Determination of CYP2D6*10 and *14 genotypes by amplification refractory mutation methods in Chinese subjects

AIM To establish methods on the basis of amplification refractory mutation polymerase chain reaction(PCR) for the genotyping of CYP2D6*10 and *14 allele in Chinese subjects.METHODS A single tube tetra-primers amplification method was used for the genotyping of CYP2D6*10 allele.An allele specific amplification method was established for the genotyping of CYP2D6*14 allele.CYP2D6 genotypes of 295 healthy Chinese subjects were analyzed.RESULTS The gene frequency of CYP2D6*10 and *14 was 55.8% and 1.8% respectively.There were 1 *14/*14,6 *1/*14 and 3 *10/*14 in 295 Chinese subjects.The CYP2D6 genotype distribution for CYP2D6*10 and *14 alleles was consistent with Hardy-Weinberg equilibrium(χ2=2.15,df=5,P0.82).CONCLUSION The genotyping method established in our laboratory is proved to be rapid and convenient,and the results are reliable.