Analysis of CYP2C19 genotypes in Chinese population using allele-specific PCR

Objective:To develop a rapid and sensitive method for genotype analysis of the CYP2C19 gene in Chinese population.Methods:The genomic DNA was extracted from lymphocytes.A common forward primer and one of the two allele-specific(AS)reverse primers were used in parallel PCR reactions.The PCR products were analyzed by electrophoresis and specific amplification indicated the presence of the allele.In order to enhance the PCR specificity,the extra mismatch based at the third or second nucleotide position from the 3'-end of AS primers were introduced.Results:Two hundred and seventy-eight DNA samples were analyzed by this new method.The CYP2C19 genotyping results derived from this ASA-PCR were perfectly consistent with sequencing.Conclusion:The new method developed and optimized in this study is a suitable strategy for CYP2C19 genotyping.It also enables accurate and rapid detection of CYP2C19 polymorphisms.