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Study on the Straw Frozen Semen of Donkey

Duan Ming-jun

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Abstract

The donkey semen was collected by artificial vagina.The extenders of frozen semen,cryoprotectant and the glycerol concentration were investigale in present study.The results shows that the lactose-glucose-glycerol-yolk extender was the best one in 5 groups(P0.05).The Glycerol was the better cryoprotective preservative compared with Glycol and DMSO.The optimum Glycerol concentration is 5%(P0.05).The sperm motility after-thawing was the highest in 5 treatment groups.The semen motility of frozen thawing was up to 0.36,Abnormal sperm after-thawing was 27.4%,acrosome integrity after-thawing was 82.2%,and sperm survival time(25℃) was8.2 h,respectively.

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What this paper is about

The donkey semen was collected by artificial vagina.The extenders of frozen semen,cryoprotectant and the glycerol concentration were investigale in present study.The results shows that the lactose-glucose-glycerol-yolk extender was the best one in 5 groups(P0.05).The Glycerol was the better cryoprotective preservative compared with Glycol and DMSO.The optimum Glycerol concentration is 5%(P0.05).The sperm motility after-thawing was the highest in 5 treatment groups.The semen motility of frozen thawing was up to 0.36,Abnormal sperm after-thawing was 27.4%,acrosome integrity after-thawing was 82.2%,and sperm survival time(25℃) was8.2 h,respectively.

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Available abstract

The donkey semen was collected by artificial vagina.The extenders of frozen semen,cryoprotectant and the glycerol concentration were investigale in present study.The results shows that the lactose-glucose-glycerol-yolk extender was the best one in 5 groups(P0.05).The Glycerol was the better cryoprotective preservative compared with Glycol and DMSO.The optimum Glycerol concentration is 5%(P0.05).The sperm motility after-thawing was the highest in 5 treatment groups.The semen motility of frozen thawing was up to 0.36,Abnormal sperm after-thawing was 27.4%,acrosome integrity after-thawing was 82.2%,and sperm survival time(25℃) was8.2 h,respectively.

Key concepts: Extender, Semen, Glycerol, Cryoprotectant, Sperm, Preservative, Sperm motility, Straw

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