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2003•The Orthopedic Journal of ChinaRequires access

Cloning and Sequencing of Bone Morphogenetic Protein 7 Gene

Yigang Tong

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Abstract

Objective:To clone BMP7 cDNA and construct the plasmid carrying BMP7 gene. Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained. The plasmids carrying BMP7 gene which was inserted T-vector were constructed. Results:Electrophoresis and sequencing showed BMP7 gene was correct. Digesting and sequencing demonstrated that the pT-BMP7 carried BMP7 gene correctly. Conclusion:The cloning of BMP7 gene can lead the basis for the further studying of BMP7 gene and protein.

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What this paper is about

Objective:To clone BMP7 cDNA and construct the plasmid carrying BMP7 gene. Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained. The plasmids carrying BMP7 gene which was inserted T-vector were constructed. Results:Electrophoresis and sequencing showed BMP7 gene was correct. Digesting and sequencing demonstrated that the pT-BMP7 carried BMP7 gene correctly. Conclusion:The cloning of BMP7 gene can lead the basis for the further studying of BMP7 gene and protein.

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Available abstract

Objective:To clone BMP7 cDNA and construct the plasmid carrying BMP7 gene. Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained. The plasmids carrying BMP7 gene which was inserted T-vector were constructed. Results:Electrophoresis and sequencing showed BMP7 gene was correct. Digesting and sequencing demonstrated that the pT-BMP7 carried BMP7 gene correctly. Conclusion:The cloning of BMP7 gene can lead the basis for the further studying of BMP7 gene and protein.

Key concepts: Bone morphogenetic protein 7, Plasmid, Gene knockin, Cloning (programming), Gene, Complementary DNA, Molecular biology, Genetics

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