Cloning of recombined human bone morphogenetic protein 2 in Escherichia coli

Objective To clone the human recombined bone morphogenetic protein 2(BMP2) gene in Escherichia coli .Methods The cell total RNA was extracted from human osteoblast cell,the BMP2 cDNA was amplified by RT PCR and then inserted into cloning vector pCI plasmid.The recombined clone was confirmed by restriction enzyme and sequencing analysis.Result Restriction enzyme assay and sequencing showed that the constructed clone contained the BMP2 cDNA.Conclusion Cloning of recombined human BMP2 gene give a basis for further expression study.