Effect of VEGF antisense oligonucleotides on growth of Lewis lung cancer

Objective To evaluate the gene therapy of lung cancer by vascular endothelial growth factor (VEGF) antisense oligonucleotide(ASON). Methods The Lewis lung cancer cells were cultured and implanted subcutaneously into 30 C57BL/6 mice. These mice were divided into three groups: VEGF-ASODN group, VEGF-SODN group,and the control group. Gene therapy were given respectively 24 hours after lung cancer cell inoculation. The weight and volume of subcutaneous tumors were measured. VEGF protein was examined by im-munohistochemistry. Partial tumor tissues were put into the liquid nitrogen and VEGF mRNA were detected by RT-PCR. Results The tumor weights of the VEGF-ASPODN group, VEGF-SPODN group, and the control group were (4.31±0.45)g, (6.47±0.71)g,and (6.23±0.76)g,respectively.The inhibition rate of tumor growth of the VEGF-ASPODN group and VEGF- SPODN group were 42.7% and 5.9% respectively. VEGF-ASODN can inhibit the expression of VEGF protein and mRNA. Conclusion It is showed that the lewis lung cancer can be inhibited by the VEGF antisense oligonucleotide inoculated into the center of the tumor in the C57BL/6 mice.