Antimicrobial activity and HPLC fingerprint of flavonoids in Flos Chrysanthemi Indici

Objective To evaluate the antimicrobial activity and establish HPLC fingerprint of flavonoids in Flos Chrysanthemi Indici.Methods Microdilution method was carried out to determine the minimum inhibitory concentration(MIC) value of total flavonoids and linarin against eleven kinds of bacteria and three fungi.HPLC fingerprint was obtained on Diamonsil C18(4.6 mm ×250mm,3 μm) with gradient elution consisting of acetonitrile(A) and 0.05% phosphoric acid(B),detective wavelength was at 326 nm and flow rate 1.0 ml / min in the room temperature.The data were analyzed by Computer Aided Similarity Evaluation software.Results MIC values of total flavonoids and linarin against Candida lusitaniae were 61.6 μg / ml and 18.0 μg / ml,respectively.Furthermore,fifteen characteristic peaks were indicated in fingerprint chromatography,and eight peaks were identified as flavonoids.Conclusion Total flavonoids and the linarin in Flos Chrysanthemi Indici exhibited better antimicrobial activity against C.lusitaiae.The established HPLC fingerprint method is accurate and reproducible,and can be used for the basic research and quality control of active flavonoids in Flos Chrysanthemi Indici.