Study on the Optimum Conditions of Extracting Total Flavonoids from Water Hyacinth

Objective:The optimum conditions of extracting total flavonoids from Water Hyacinth(Eichhornia crassipes) were studied.Method:By extracting the total flavonoids from Water Hyacinth with the two different methods of extraction and ultrasonic and taking Ethanol as menstruum the two extracting methods were compared;The activity of anti-oxygen of flavonoids extract was also studied.Result:Under the temperature of 70℃,50% of Ethanol concentration extracting with 1∶40 feed ratio in 3h were the best conditions of extracting total flavonoids,4.03% was the extraction ratio of the total flavonoids.With 320W ultrasonic power,50% of Ethanol concentration extracting with 1∶40 feed ratio in 30 min were the best conditions of extracting total flavonoids.5.09% was the extraction ratio of the Water Hyacinth total flavonoids under the circumstances;The Water Hyacinth total flavonoids were better than Mannitol in eliminating ·OH while the abilities were lower than ascorbic acid(Vc) in getting rid of ·O2-.Conclusion:Ultrasonic was considered the more desirable way to extract flavonoids and the Water Hyacinth flavonoids were considered certain anti-oxygen activity.