Effect of Vernoniaanthelmintica willd on tyrosinase activity and melanogenesis in A375human melanoma

To study the effect of Vernoniaanthelmintica willd,on cell proliferation tyrosinase activity and the melanogenesis of A375human melanoma cell.Tyrosinase activity was measured by zymologic method with its absorption at475nm.MTT was used to study the proliferation of A375human melanoma cell.The melanin was detected by its absorption at475nm.Vernoniaanthelmintica willd enhanced significantly the synthesis of tyrosinase,cell proliferation and the melanogenesis of A375human melanoma cell in vitro.Effect of Vernoniaanthelmintica willd treating vitiligo is relative with enhancing activity of tyrosinase and melanogenesis