2002•Zhongguo yaolixue tongbaoRequires access
Effect of hydrocortisone injection on tyrosinase activity and melanogenesis of B-16 murine melanoma cells
Deng Rui
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Abstract
AIM To study the effect of Hydrocortisone injection on tyrosinase activity and the melanogenesis of B 16 murine melanoma cell. METHODS Tyrosinase activity was measured by zymologic method with its absorption at 490 nm. MTT was used to study the proliferation of B 16 murine melanoma cells. The melanin was detected by its absorption at 570 nm. RESULTS Hydrocortisone injection can significantly enhance the activity of tyrosinase in vitro and of mice serum and enhance the synthesis of tyrosinase, the melanogenesis, the proliferation of B 16 murine melanoma cells. CONCLUSION Hydrocortisone injection activates the activity of tyrosinase in vitiligo treatment.