2007•Chinese Journal of Aesthetic MedicineRequires access
Effects of hydyocortisone on the melanogenesis in A375 human melanoma cells
Zhijun Liu
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Abstract
Objective To observe the effects of hydrocortisone in different concentration on the cell growth,melanogenesis and tyrosinase activity in A375 human melanoma cells for dissecting the mechanism of hydrocortisone in treating vitiligo.Methods After the A375 melanoma cells were dealed with hydrocortisone in different concentrations,we observed the growth of A375 melanoma cells by MTT assay;measured the contents of melanin by NaOH lysis method;and detected the change of tyrosinase activity by dioxyphenylalanine oxidation.Results Compared with the control group,hydrocortisone concentration in a certain extent enhanced the proliferation of A375 human melanoma cells,tyrosinase activity and melanogenesis.Conclusion Hydrocortisone of certain concentration can promote the proliferation of A375 human melanoma cells and activate the activity of tyrosinase,enhence the melanogenesis,and the experiment has provided an experimental basis for exploring the mechanism of hydrocortisone in treating vitiligo.