Study on plasma concentration assay and bioavailability of freeze - dried albendazole liposomes

Objective To establish a HPLC method for the determination of albendazole and its metabolites in rat plasma,and then calculate the relative bioavailability of freeze- dried albendazole liposomes( L-ABZ).Methods A single oral dose of freeze- dried L- ABZ and albendazole tablets( T- ABZ) were given in rats,plasma concentrations at each time point were assayed by HPLC method.The pharmacokinetic parameters were calculated with 3P97 program.Results The pharmacokinetic parameters of two formulations were as follows: Cmaxwere( 4.32 ±0.70),( 5.27 ± 0.60) μg ·m L- 1; Tmaxwere( 4.71 ± 1.17),( 5.39 ±0.94) h; AUC were( 63.93 ± 13.08),( 84.56 ± 14.97) μg·h·m L- 1,respectively.Compared with the T- ABZ,the relative bioavailability of freeze- dried L- ABZ was 154.17%.Conclusion The pharmacokinetics of two formulations accord with the two compartment models and the freeze- dried can improve bioavailability significantly.