Isolation and culture of hunman uterine leiomyoma cells in vitro and expression of estrogen and progesterone receptor

OBJECTIVE:To study is how to get the uterine leiomyoma cells in vitro which have the similar bionomics to the leiomyoma cells in human body,and is to discuss the senses of ER,PR expressions in the uterine leiomyoma tissues,normal autologous unaffected myometrium and the cultured uterine leiomyoma cells in vitro.METHODS:Human uterine leiomyoma tissues were dissected and cultured.Characterization of the cultured cells was examined using immunostaining with monoclonal antibodies to a muscle-specific protein desmin.RT-PCR was used to detect the mRNA expression of ER,PR in the cultured leiomyoma cells,uterine leiomyoma tissues and the normal autologous unaffected myometrium.RESULTS:The uterine leiomyoma cells were got by using collagenase dissection and were confirmed by immunostaining with monoclonal antibodies to a muscle-specific protein desmin.RT-PCR analysis revealed that ER,PR mRNA expressions have no significant changes between the cultuled cells(ER 0.92±0.24;PR 0.62±0.14) and the leiomyoma tissues(ER 0.91±0.30;PR 0.60±0.11;P0.05),however ER,PR mRNA expressions in the normal autologous unaffected myometrium(ER 0.64±0.31;PR 0.46±0.16) were decreased to compare with the cultured cells and the tissues(P0.05).CONCLUSION:The uterine leiomyoma cells can be got through the method of collagenase dissection which have the similar bionomics to the leiomyoma cells in human body in the genes of ER,PR expression.