Partitional Behaviors of Flavones of Persimmon Leaves in Two-phase Aqueous System
LI Xiao-yin
Abstract
LI Xiao-yin
Abstract
Effective extraction of flavones from persimmon leaves is an important technique in bioresearch.In the present study,extraction and purification of flavones of persimmon leaves were carried out simultaneously by using an aqueous partitioning(method.) This two-phase aqueous system was prepared by dissolving suitable amounts of polyethylene glycol(PEG)and(NH_4)_2SO_4 in(aqueous) solution.Flavones of persimmon leaves were recovered with optimum high yield and high concentration in the upper(polymer-rich) phase when(25%) PEG 600and(25%)(NH_4)_2SO_4 were applied.The optimum extraction conditions were pH value 11,MgCl_2 3% and temperature 25℃.
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Effective extraction of flavones from persimmon leaves is an important technique in bioresearch.In the present study,extraction and purification of flavones of persimmon leaves were carried out simultaneously by using an aqueous partitioning(method.) This two-phase aqueous system was prepared by dissolving suitable amounts of polyethylene glycol(PEG)and(NH_4)_2SO_4 in(aqueous) solution.Flavones of persimmon leaves were recovered with optimum high yield and high concentration in the upper(polymer-rich) phase when(25%) PEG 600and(25%)(NH_4)_2SO_4 were applied.The optimum extraction conditions were pH value 11,MgCl_2 3% and temperature 25℃.
Key concepts: Flavones, Chemistry, Polyethylene glycol, Extraction (chemistry), Aqueous solution, PEG ratio, Dissolution, Chromatography