Determination of Anthranilic Acid and Syringic Acid in Folium Isatidis by RP-HPLC

Objective To establish a RP-HPLC method to determine the contents of anthranilic acid and syringic acid in Folium Isatidis. Methods Chromatography was carried out on a Lichrosorb C_(18) column(4.6 mm×150 mm,5 μm);mobile phase: acetonitrile-water-phosphoric acid(18∶82∶0.3);flow rate: 0.8 mL·min~(-1);column temperature:30 ℃,sample volume:10 μL;and the detection wavelength was 272 nm. Results A good linearity of anthranilic acid was obtained within 12.7-(127.0) μg·mL~(-1)and syringic acid was within 5.10-51.0 μg·mL~(-1).The average recoveries of anthranilic acid was(98.7%)(RSD=(1.0%));and syringic acid was 99.0%(RSD=1.2%),respectively. Conclusion The method is sensitive,rapid,and accurate.