2014•Xinjiang Yike Daxue xuebaoRequires access

The determination of the content of luteolin in Kunlun chrysanthemum by HPLC

Zhao Wenhu

Abstract

Objective To develop an HPLC method for the determination of the content of luteolin in Kunlun chrysanthemum.Methods The content of luteolin in Kunlun chrysanthemum was determined by HPLC.The exact process was:column:Waters XBridge C18(250mm×4.6mm,5μm);mobile phase A: cetonitrile;B:2%glacial acetic acid(V/V),gradient elution:0min,A∶B(15∶85);10min,A∶B(20∶ 80);15min,A∶B(35∶65);30min,A∶B(15∶85);the flow rate was 1.0mL/min,the detection wavelength was at 350nm,and the column temperature was 35℃.Results Linearity of luteolin was good in the range of 0.1~1μg/mL(r=0.999 9).The average recovery of luteolin was 94.90% with RSD 0.69%. Conclusion This method is convenient,accurate,and can be used to determine the content of luteolin in Kunlun chrysanthemum.

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Key concepts: Luteolin, High-performance liquid chromatography, Chromatography, Chemistry, Content determination, Gradient elution, Acetic acid, Elution