Analysis of cellular and humoral immunity function in myelodysplastic syndrome patients

Objective To evaluate the changes and significance of cellular and humoral immunity levels of patients with myelodysplastic syndrome(MDS).Methods Flow cytometry was adopted to examine peripheral blood lymphocyte subpoplulations ratios among 30 healthy volunteers and 31 MDS patients(low,medium and high risk groups),the patients were confirmed in diagnosis but not yet treated.The ratios of lymphocyte subpoplulations included CD3+ T cells and its subpoplulations,CD3-CD19+ B cells and CD3-(CD16CD56)+ natural killer cells(NK) subsets.Results Compared with those of healthy controls,T cells,B cells and NK cells counts of peripheral blood in MDS patients were obviously decreased.In addition,among T lymphocyte subpoplulations,the expressions of CD3+CD4+ helper T cells(Th) in MDS patients were lower,the expressions of CD3+CD8+ T suppressor cells(Ts) in MDS patients were higher,and the proportion of Th and Ts was inverted,and the ratio of subpopulation was imbalanced.With the progression of the disease,the expressions of Ts cells were gradually increasing.But the differences among three risks′ groups were not statistically significant.The expressions of NK cells were gradually decreasing.But the differences among the three groups were not statistically significant(P0.05).The differences of T cells and Th/Ts in three risks′ groups were not statistically significant.Conclusion Both cellular and humoral immunity function in MDS patients decreased.The examination of lymphocyte subpopulations in peripheral blood can be used to assess the immune function of patients with MDS.