Procyanidin induces MCF-7 cell apoptosis by Caspase way

Objective To investigate the mechanism that procyanidin induced apoptosis of human breast cancer cells and the apoptosis pathway,and to reveal part of the mechanism of its anti-breast cancer.Methods Cells were cultivated in vitro,some good cells were taken and divided into positive control group,negative control group and PC group randomly after cultured for 24 h.The cytostatic effect of procyanidin on human breast cancer cell line MCF-7 was evaluated by MTT assay.Flow cytometry was utilized for measuring the apoptosis and cell cycle.The expressions of Caspase-9,Caspase-12 in cultured supernatant were detected by ELISA assay.Results Procyanidin inhibited cell growth in a concentration-and time-dependent manner.At the dosage of 40,80 and 160 μg/mL in cultured cells,the apoptosis rates was elevated,and G2-M phrase was arrested.Significant up-regulation of Caspase-9,Caspase-12 was observed in MCF-7 cells 48 h after treatment with procyanidin.Conclusion Procyanidin inhibited the proliferation of MCF-7 cells associated with cycle arrest in G2-M phase,induction of apoptosis.Caspase pathway was related in the apoptosis pathway,moreover,endoplasmic reticulum and mitochondria were involved in the Caspase family.