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Effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-α

Huang Di-nan

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Abstract

Objective To investigate the effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-α. Methods Flow cytometry was used to analyze the apoptosis. The caspase-8 fluorescent kits were used to detect the activity of caspase-8. And the expression of caspase-8 enzymogen was examined by Western blot. Results Procyanidin (5, 10, and 20 mg/L) significantly inhibited the apoptosis of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner; procyanidin (5, 10, and 20 mg/L) had significantly inhibition on the caspase-8 activation of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner. Procyanidin (5, 10, and 20 mg/L) had no effect on the expression of caspase-8 enzymogen of HeLa cells. Conclusion The inhibition of procyanidin on apoptosis of HeLa cells induced by rhTNF-α may have relation to the inhibition of caspase-8 activation.

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Objective To investigate the effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-α. Methods Flow cytometry was used to analyze the apoptosis. The caspase-8 fluorescent kits were used to detect the activity of caspase-8. And the expression of caspase-8 enzymogen was examined by Western blot. Results Procyanidin (5, 10, and 20 mg/L) significantly inhibited the apoptosis of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner; procyanidin (5, 10, and 20 mg/L) had significantly inhibition on the caspase-8 activation of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner. Procyanidin (5, 10, and 20 mg/L) had no effect on the expression of caspase-8 enzymogen of HeLa cells. Conclusion The inhibition of procyanidin on apoptosis of HeLa cells induced by rhTNF-α may have relation to the inhibition of caspase-8 activation.

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Available abstract

Objective To investigate the effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-α. Methods Flow cytometry was used to analyze the apoptosis. The caspase-8 fluorescent kits were used to detect the activity of caspase-8. And the expression of caspase-8 enzymogen was examined by Western blot. Results Procyanidin (5, 10, and 20 mg/L) significantly inhibited the apoptosis of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner; procyanidin (5, 10, and 20 mg/L) had significantly inhibition on the caspase-8 activation of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner. Procyanidin (5, 10, and 20 mg/L) had no effect on the expression of caspase-8 enzymogen of HeLa cells. Conclusion The inhibition of procyanidin on apoptosis of HeLa cells induced by rhTNF-α may have relation to the inhibition of caspase-8 activation.

Key concepts: HeLa, Apoptosis, Flow cytometry, Caspase 3, Chemistry, Western blot, Molecular biology, Proanthocyanidin

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