Establishment and identification of human gastric epithelial cell strain GES-1 stably transfected by BCL-2 gene

Objective To establish a Human Gastric Epithelial cell strain GES-1stably transfected by BCL-2 gene.Methods After the pc~(DNA3-BCL-2)was identified by restriction endocleavase digestion,lipofectamine method was adopted to transfect pc~(DNA3-BCL-2)into human gastric epithelial cell line GES-1.The geneticin 418(G418) was used to select positive clones.FITC labeled immunohistological staining was used to detect BCL-2 protein positively expressed cells.Results Identified by restriction endocleavase digestion,it was the plasmid carrying BCL-2 gene.BCL-2 protein positive expression in 7 transfected cell clones were identified by FITC labeled immunohistological staining.BCL-2 protein was expressed in cytoplasm and nucleomembrane.Conclusion These cells are BCL-2 protein positively expressed cells.The establishment of this cell model can be used to further study of the role of(BCL-2) gene in gastric carcinoma.