Establishment of regeneration system from leaf explant of Dianthus caryophyllus

The regeneration system from leaf of Dianthus caryophyllus Linn. was established by using different cytokinins combining with other phytohormones. The results showed that the kinds of cytokinin effected the differentiation rate of adventitious buds from leaf; 6-BA and TDZ in relatively low concentration can induce differentiation of adventitious buds from leaf and PP_333) can increase the differentiation rate and the number of adventitious buds. The system of plant regeneration from leaf of D. caryophyllus consisted of: MS + 0.002 mg·L~(-1) TDZ + 0.5 mg·L~(-1) 6-BA + 0.2 mg·L~(-1) IAA+4 mg·L~(-1) PP_333) for the differentiation of adventitious buds, MS + 0.2 mg·L~(-1) 6-BA+ 0.2 mg·L~(-1) IAA for the growth of adventitious buds and 1/2 MS for the rooting of adventitious buds. The differentiation rate of adventitious buds is 42.61% and the average number of adventitious bud is 4.53.