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Study on direct regenerating adventitious buds from different organs of grape

Quan Dongling, Chang Yong-yi

Open publisher page 3 citations

Abstract

Direct organs regeneration system was studied using the leaves, petioles, stems and the roots of test-tube plantlets of grape .The results indicated that the best medium for regeneration were MS medium and the best explant was leaf. The optimum medium for inducing adventitious buds from leaves as MS + 6-BA (1.5～2.0 mg/L) + IBA(0.01～0.1 mg/L), and the leaves of the cultivars such as Red Globe, Victoria and GN-18 produced higher adventitious buds. Within the concentration scope of BA from 1.5 to 2.0 mg/L, higher α-naphthalene acetic was unfavourble to produce directly adventitious bud from organs.

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What this paper is about

Direct organs regeneration system was studied using the leaves, petioles, stems and the roots of test-tube plantlets of grape .The results indicated that the best medium for regeneration were MS medium and the best explant was leaf. The optimum medium for inducing adventitious buds from leaves as MS + 6-BA (1.5～2.0 mg/L) + IBA(0.01～0.1 mg/L), and the leaves of the cultivars such as Red Globe, Victoria and GN-18 produced higher adventitious buds. Within the concentration scope of BA from 1.5 to 2.0 mg/L, higher α-naphthalene acetic was unfavourble to produce directly adventitious bud from organs.

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Available abstract

Direct organs regeneration system was studied using the leaves, petioles, stems and the roots of test-tube plantlets of grape .The results indicated that the best medium for regeneration were MS medium and the best explant was leaf. The optimum medium for inducing adventitious buds from leaves as MS + 6-BA (1.5～2.0 mg/L) + IBA(0.01～0.1 mg/L), and the leaves of the cultivars such as Red Globe, Victoria and GN-18 produced higher adventitious buds. Within the concentration scope of BA from 1.5 to 2.0 mg/L, higher α-naphthalene acetic was unfavourble to produce directly adventitious bud from organs.

Key concepts: Explant culture, Biology, Regeneration (biology), Botany, Cultivar, Horticulture, In vitro, Biochemistry

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