Surveillance of Bacterial Resistance in Clinical Isolates from Xiangya Hospital

Objective To investigate the distribution and antibiotic resistance of clinical isolates collected from Xiangya Hospital of Central South University in 2009. Methods The isolates and their susceptibilities were determined by automatic microorganism analytical system VITEK-2.All the data were analyzed by WHONET 5.4 software. Results A total of 7,841 isolates were collected,of which gram positive cocci,gram negative organisms and fungus accounted for 28.5%(2,237/7,841),55.7% (4,369/7,841),and 15.8%(1,235/7,841),respectively.The first 5 gram positive cocci were coagulase negative Staphylococcus(CNS),Staphylococcus aureus,Enterococcus faecalis,Enterococcus faecium and Streptococcus sanguis.Methicillin resistant strains in S.aureus(MRSA) and coagulase negative Staphylococcus(MRCNS) accounted for 63.7% and 67.2%,respectively.No staphylococcal strain was intermediate or resistant to vancomycin.Seven strains of Enterococcus spp.were resistant to vancomycin(VRE).The first 5 gram negative bacilli were Pseudomonas aeruginosa,Escherichia coli,Acinetobacter baumannii,Klebsiella pneumonia,and Enterobacter cloacae.The strains of Enterobacteriaceae,like E. coli,K.pneumonia,and E.cloacae,had very low resistance rates to carbopenems(2.3%).The resistance rates of E.coli and K.pneumonia to piperacillin/tazobactam or cefoperazone/sulbactam were less than 13.0%.The resistance rates of E.cloacae to piperacillin/tazobactam and cefoperazone/sulbactam were 40.0% and 28.4%,respectively.Among non-fermenter,the resistance rates of P.aeruginosa to meropenem and cefoperazone/sulbactam were 9.1% and 12.4%,respectively.The resistance rates of A.baumannii to cefoperazone/sulbactam,levofloxacin and and ampicillin/ sulbactam were 7.0%,7.6% and 16.3%,respectively.The resistance rate of A.baumannii to meropenem was high(28.8%) and was even higher to other commonly used antibiotics(40%). Conclusions Gram negative bacilli are still the major isolates and multidrug resistance is serious in our hospital.Clinical doctors should pay special attention to the increasing strains of vacomycin resistant Enterococcus spp. and carbapenems-resistant non-fermenter.It is urgent that effective control measures should be taken to prevent dissemination and outbreak of these stains.