Assessment of the hemoglobin detection in the diagnosis of the thalassemia

Objective To assess the hemoglobin analysis in the diagnosis of the thalassemia. Methods The hemoglobin detection was performed using HLC1,723G7 analyzer in 115 patients diagnosed as thalassemia based on the clinical manifastations, the epidemiological findings and cellulose acetate membrane electrophoresis1,elution. The diagnostic significance was assessed in combination with the DNA analysis. Results The patients with higher HbA2 value than 5.5% were conformed to 81.5% β1,thalassemia diagnosed through the DNA analysis. The patients with lower HbA2 value than 2.5% were conformed to 92.5% α1,thalassemia diagnosed through the DNA analysis. Conclusions The diagnosis of α or β1,thalassemia may be confirmed through the automated hemoglobin analysis, the clinical manifestations and the DNA analysis.