The expression of bc1-2 gene in hippocampus area of neonatal rats with hypoxic ischemic brain damage

Objective To explore the effect of bcl-2gene in cell apoptosis of neuron by studying the expression of bc1-2 gene in hippocampus area of neonatal rat with hypoxic ischemic brain damage(HIBD).Methods Fifty-four neonatal rats were stochastically divided into 1 control group and 8 trial groups.The HIBD in neonatal rat were established by inhaling the mixed gases of 92%N_2 and 8%O_2,then the animals were sacrificed by dislocating their heads at different time points(0.5,1.0,3.0,6.0,12.0,24.0,48.0,72.0h),and the hippocampus tissue were taken out.The brain nerve cell apoptosis and the expression of bc1-2 gene were detected.Results The pathology change of the positive apoptosis cells in every group's rats are that the chromatin shrank and collected,the cytoplasm condensed and there are densely dyed grain.The apoptotic cells appeared at the time point of 3h,and reached the peak at 48 h,then decreased.the positive cell of bcl-2 protein increased from the time point of 30 min and reached the peak at 6h and then decreased gradually following HIBD.Conclusion The expression of bc1-2 gene in hippocampus was stronger than other areas of brain,and there is some relativity between cell apoptosis and exprssion of bc12 gene.