Surveillance on bacterial resistance in Shenzhen Nanshan Hospital in 2010

Objective To investigate the resistance of clinical bacterial isolates from a hospitals in 2010.Methods Antimicrobial susceptibility of bacterial isolates from inpatients was detected by BD-automatic identification of bacterial analyzer,and data were analyzed with WHONET5.4 software.Results A total of 2 192 pathogenic strains were isolated from various clinical specimens from January to December,2010,67.97% of which was gram-negative bacilli and 32.03% was gram-positive cocci.19.69% of Staphylococcus aureus and 54.59% of coagulase negative Staphylococcus was methicillin-resistant(MRSA and MRCNS respectively).The resistant rates of methicillin-resistant strains to β-lactams and other antimicrobial agents were much higher than those of methicillin-sensitive strains.The resistant rate of MRSA to sulfamethoxazole/trimethoprim,rifampin,tetracycline,and gentamicin was 1.67%,41.54%,44.62%,and 58.46%,respectively,the resistant rates of MRCNS to rifampin and tetracycline was 17.27% and 36.70%,respectively;vancomycin-,teicoplanin-and linezolid-resistant strain was not found.The resistant rates of Enterococcus faecalis to most detected antimicrobial agents were much lower than those of Enterococcus faecium.One linezolid-resistant Enterococcus faecalis isolate was first reported in this hospital,vancomycin-resistant strain was not found.Extended-spectrum β-lactamase(ESBL)-producing strains accounted for 44.29% of Escherichia coli and 15.79% of Klebsiella pneumoniae.Resistant rates of ESBL-producing Enterobacteriaceae strains were higher than non-ESBL-producing Enterobacteriaceae strains.Resistant rate of Pseudomonas aeruginosa to imipenem and meropenem was 26.73% and 13.79%,respectively,resistant rate of Acinetobacter spp.(92.91% were Acinetobacter baumannii) to above two carbapenems was 31.35% and 27.17%,respectively.Conclusion Bacterial resistance is on the rise,especially drug resistance of gram-negative bacilli;resistance of Acinetobacter baumannii and Pseudomonas aeruginosa to carbapenems is increasing.It is important to use antimicrobial agents rationally,detect pandrug-resistant strains early,and strengthen infection control.