Relationship between rennin and angiotensinogen gene polymorphisms and cerebral infarction

Objective To study the relationship between the renin (REN) gene G10631A, angio-tensinogen (AGT) gene T704C, C521T mononucleotide polymorphisms and cerebral infarction. Methods One hundred eighty patients with cerebral infarction and 130 healthy controls were recruited. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the REN G10631 site, AGT T704 and C521 site genotype and allele.The differences of the genotype and allele frequencies in both groups were compared.Logistic regression was used to analyze the risk factors for cerebral infarction.The haplotype structure of the population was analyzed in order to find cerebral infarction related polymorphism combination in this population.Results ①The REN 10631 AA genotype frequency (31.7%) and the A allele frequency (49.4%) in the cerebral infarction group were higher than 10.0% and 30.3% in the healthy control group (P 0.05).②The AGT 704 CC genotype frequency (63.3%) and C allele frequency (79.7%) in the cerebral infarction group were higher than 34.6% and 61.2% in the healthy control group (P 0.05).③The AGT 521IT genotype frequency (21.7%) and T allele frequency (27.8%) in the cerebral infarction group were higher than 6.9% and 11.9% in the healthy control group (P 0.05).④Multivariate Lo gistic regression analysis showed that REN 10631AA genetype, AGT 704CC gene- type, and AGT521TT genetype could increase the probability of cerebral infarction.The relative risk (OR) of the onset was 2.617 , 2.699 , and 3.362, respectively (P 0.05) ; ⑤The distribution frequency of the haplotype 521T-10631A-704C in the cerebral infarction group was higher than that in the healthy control group (P=0.000).Conclusion REN gene 10631AA genetype and A allele, AGT 704CC genetype and C allele, and AGT 521TT genetype and T allele may be the susceptible factors of cerebral infarction.Haplotype 521 T-10631A-704C may be the genetic risk factors for the onset of cerebral infarction.