Effect of manganese chloride on PC12 apoptosis and transmitochondrial membrane potential

Objective To research the toxic effects on manganese chloride(MnCl2) of apoptosis and mitochondrial transmembrane potential of pheochromocytoma (PC12) cells and to reveal the molecular mechanisms of dysfunction caused by manganese.Methods PC12 cells in logarithm period were incubated in culture media of MnCl2 at 100,300,500,700,900 μmol/L for 24,48 and 72 hours respectively,cell viability was examined by MTT.Cell cycle,apoptosis and mitochondrial transmembrane potential were monitored by flow cytometry (FCM).Results Compared with the control group,24 hours after the incubation,the cell inhibition rates only in 700 and 900 μmol/L MnCl2 groups were significantly increased (P0.05),while at 48 and 72 hours,cells inhibition rates at every MnCl2 group were significantly increased (P0.05).At 48 hours,the results of 500,700 and 900 μmol/L MnCl2 group showed that,compared with the control group,G1 phase was increased,S phase was decreased,the percentage of G2/M and apoptosis were increased (P0.05) respectively.The cell membrane potential was decreased compared with control group.Conclusion MnCl2 could bring down mitochondrial transmembrane potential of PC12 cells and inhibit multiplication of cells and induce cell apoptosis.