Development of the standard curve for detecting the expression of human glucocorticoid receptor-α mRNA with real time fluorescence quantitative RT-PCR

The standard curve for detecting human glucocorticoid receptor alpha(GR-α) mRNA expression with fluorescence quantitative polymerase chain reaction was developed.The probe and primers were designed and synthesized according to the human GR-α(GeneBank No.NM_000176) mRNA sequence.Then a TaqMan probe fluorescence quantitative RT-PCR assay was carried out,and the standard curve for detecting human GR-α mRNA expression was constructed.The standard curve made by pMD18–T–GR-α had good linear dependence,and it was sensitive(it could detect 10 copies/μL of plasmid DNA) and specific.The standard curve for detecting human GR-αmRNA expression with fluorescence quantitative RT-PCR was developed successfully.