2006•Biotechnology(Faisalabad)Requires access

Development of the Standard Curve for Detecting Pig IGF-I mRNA with Fluorescence Quantitative Polymerase Chain Reaction

Xiao Shu-qi, Shumin Zhang

Abstract

Objective:The standard curve for detecting pig IGF-ⅠmRNA expression with fluorescence quantitative polymerase chain reaction was developed.Methods:The probe and primers were designed and synthesized according to the pig IGF-Ⅰ(GenBank No.DQ784687)mRNA sequence.Then a Taqman probe fluorescence quantitative RT-PCR assay was carried,and the standard curve for detecting pig IGF-ⅠmRNA expression was constructed.Results:The standard curve made by pMD-18T IGF-Ⅰhad good linear dependence,and it was sensitive(it could detect 10 copies/μL of plasmid DNA) and specific.Conclusion: The standard curve for detecting pig IGF-ⅠmRNA expression with fluorescence quantitative RT-PCR was developed successfully.

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Available abstract

Key concepts: Standard curve, TaqMan, Real-time polymerase chain reaction, Molecular biology, Messenger RNA, Polymerase chain reaction, GenBank, Biology