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2006•Zhongguo shouyi kexueRequires access

Development of a fluorescent quantitative RT-PCR assay for detection of expression of leptin mRNA

Zhe Wang

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Abstract

The probe and primers were designed and synthesized according to the leptin sequence available in GenBank. Then a real-time RT-PCR assay was developed and assessed. Results showed that the standard curve made by pMD-18T leptin had good linear dependence, and the fluorescent quantitative RT PCR assay was sensitive (it could detect 10 copies/μL of plasmid DNA) and specific.

About this research paper

What this paper is about

The probe and primers were designed and synthesized according to the leptin sequence available in GenBank. Then a real-time RT-PCR assay was developed and assessed. Results showed that the standard curve made by pMD-18T leptin had good linear dependence, and the fluorescent quantitative RT PCR assay was sensitive (it could detect 10 copies/μL of plasmid DNA) and specific.

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Available abstract

The probe and primers were designed and synthesized according to the leptin sequence available in GenBank. Then a real-time RT-PCR assay was developed and assessed. Results showed that the standard curve made by pMD-18T leptin had good linear dependence, and the fluorescent quantitative RT PCR assay was sensitive (it could detect 10 copies/μL of plasmid DNA) and specific.

Key concepts: Real-time polymerase chain reaction, Leptin, GenBank, Standard curve, Molecular biology, Messenger RNA, Fluorescence, Plasmid

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