Detection and Evaluation of Antibiotic Susceptibility of Ureaplasma Urealyticum and Mycoplasma Hominis in Shiyan Area and Associated Mechanisms of Drug Resistance to Tetracycline

Objective To investigate the infectious status in urogenital tract and changing trend of drug susceptibility of mycoplasma in Shiyan area and associated mechanisms of drug resistance to tetracycline.Methods U.urealyticum(Uu) and M.hominis(Mh) were detected in 1 568 cases with urogenital tract infection,and the susceptibility of clinical strains to antibiotics were determined using Mycoplasma IST kit and based on the susceptibility tested,the resistance gene tetM from strains of urealyticus were amplified by PCR.Results The total positive rate of mycoplasma infection in female was 43.5%,447 cases were infected by U.urealyticum,and 88 cases by M.hominis and 147 cases were positive with both species.Doxymycin,Josamycin and Minomycin were the most effective agents against Uu strains.The azitromycin and ofloxacin showed a high resistance rate.The resistance strains of U.urealyticum to tetracycline possessed the resistance gene tetM.Conclusion Mixed infection and increasing resistance of mycoplasmas is serious.Multiple resistance to two or more antibiotics are common.Mycoplasma is a predominant pathogen in female genitourinary tract infection,and it is sensitive to Doxymycin,Josamycin and Minomycin.This findings also indicate that resistance monitoring of mycoplasma periodically has important significance in clinical practices.