Reversal Effect of Matrine on Multidrug Resistance in Human Gastric Cancer Cell Line SGC7901/VCR

Objective To explore the reversal effect of matrine on multidrug resistance(MDR) in drug-resistant human gastric cancer cell line SGC7901/VCR and its mechanism.Methods The reversal effect of matrine on proliferation of multidrug-resistant SGC7901/VCR cells was determined by using MTT assay,and the expression of MDR-associated protein(MRP) in human gastric cancer cells was detected by flow cytometry.Results The growth inhibition rates of SGC7901/VCR cells were 10%,15% and 32% after 72 hours of treatment with matrine at concentrations of 0.5,1,and 2 mg·mL-1,with reversal of 9.4,13.4,24.5 fold,respectively.The expression of MRP was decreased from(36.00±4.32)% to(25.00±3.85)% after 24 hours of treatment with 0.5ug/mL matrine(P0.01).Conclusion Matrine can reverse MDR of human gastric cancer cell line SGC7901/VCR in part by a mechanism involving decrease of MRP expression.