2008•International Journal of Digestive DiseasesRequires access

Reversal of multidrug resistance in drug-resistant human gastric cancer cell line SGC7901/VCR by verapamil

Kang Chunsheng

Abstract

Objective To explore the reversal effect of verapamil on multidrug resistance(MDR) in drug-resistant human gastric cancer cell line SGC7901/VCR and its mechanisms.Methods Expression of multidrug resistance 1(MDR1) gene was detected using reversal transcription-polymerase chain reaction(RT-PCR).Expression of P-glycoprotein(P-gp) was detected by immunohistochemistry method.Tetrazolium blue(MTT) assay was used to evaluate the sensitivity of cells to anticancer agents,mitomycin and tegafur.Results Expression of MDR1 gene and P-gp were knockdown by verapamil.MTT showed that the sensitivity to anticancer agents were promoted by verapamil.Conclusion Verapamil has potent reversal effect on MDR in SGC7901/VCR via inhibiting the function of MDR1 and P-gp,and enhancing the sensitivity to anticancer agents,mitomycin and tegafur.

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Key concepts: Verapamil, Multiple drug resistance, MTT assay, Mitomycin C, P-glycoprotein, Pharmacology, Drug resistance, Paclitaxel