Experimental study on reversion effect of elemene on multidrug resistance of human gastric cancer cell line

Objective To observe the reversion effect of elemene on human drug-resistant gastric cancer cell line SGC_ 7901 /VCR. Methods The cell cycle alteration,intracellular drug concentration of these cells,the sensitivity of gastric cancer cells to several anti-tumor drugs and expression of P-gp and MRP in gastric cancer cells were detected by FCM,MTT assay and immunocytochemical staining,respectively.Results The IC50 value of elemene to SGC_ 7901 /VCR was 18.65μg/mL. Elemene could reserve the multidrug resistance(MDR) of SGC_ 7901 /VCR to VCR and ADM.but SGC_ 7901 /VCR sensitivity to 5-FU and DDP had no obvious change.By comparing with SGC_ 7901 /VCR, SGC_ 7901 /VCR with P-gp and MRP cells decrease in the G1 stage and increase in the S and G2+M stage. P-gp and MRP expression level was lower than that without elemene. Conclusion Elemene could reserve the multidrug resistance(MDR) of SGC_ 7901 /VCR,for which the decreased expression of P-gp and MRP maybe responsible.