Improvement of Quality Standards of Genuine Regional Drug Angelicae Dahuricae Radix from Sichuan Province

OBJECTIVE:To solve the problems of non-standard specification and quality differences of Angelicae Dahuricae Radix due to the reason of original processing,and to improve quality standards of Angelicae Dahuricae Radix from Sichuan province.METHODS:Using oxypeucedanin,imperatorin and isoimperatorin as index,the quality standards of Angelicae Dahuricae Radix either sulfured or not were established by TLC and HPLC.RESULTS:The characteristic spots of oxypeucedanin,imperatorin and isoimperatorin of 12 batches of Angelicae Dahuricae Radix were detected by TLC.The linear ranges of oxypeucedanin,imperatorin and isoimperatorin were 0.003 08-0.061 54 mg/ml(r=0.999 9),0.003 74-0.074 88 mg/ml(r=0.999 9)and 0.002 90-0.057 60 mg/ml(r=0.999 9)separately.The recoveries were 100.00%(RSD=1.80%,n=6),99.39%(RSD=1.11%,n=6) and 99.81%(RSD=1.41%,n=6),separately.CONCLUSION:TLC identification is strong specific;and HPLC is high accurate,good reproductive,convenient and rapid,which could be applied to evaluate whether Angelicae Dahuricae Radix sulfured or not and determine effective components,so as to carry out a more scientific method for the quality control of Angelicae Dahuricae Radix.