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Determination of contents of imperatorin and isoimperatorin in Radix Angelicae Dahuricae from different areas by HPLC.

Hui Zhang, Hai GuangFan, Yin ZhiKui

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Abstract

Objective To develop a method for the determination of imperatorin and isoimperatorin in Radix Angelicae Dahuricae.Methods A HPLC method was established.Lichrospher-C18 column(250 mm×4.6 mm,5 μm)was used,with mixture of methanol and water in a gradient mode.The flow rate was 1.0 mL·min-1.Results The calibration of imperatorin was linear in range of 0.54～5.40 μg.The average recovery was 99.020%,RSD 3.318%.The calibration of isoimperatorin was linear in range of 0.244～2.44 μg.The average recovery was 97.528%,RSD 2.904%.The average contents of imperatorin and isoimperatorin were 1.465 mg·g-1 and 0.575 mg·g-1 respectively.Conclusion The method is appropriate for the detemination of Radix Angelicae Dahuricae.

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What this paper is about

Objective To develop a method for the determination of imperatorin and isoimperatorin in Radix Angelicae Dahuricae.Methods A HPLC method was established.Lichrospher-C18 column(250 mm×4.6 mm,5 μm)was used,with mixture of methanol and water in a gradient mode.The flow rate was 1.0 mL·min-1.Results The calibration of imperatorin was linear in range of 0.54～5.40 μg.The average recovery was 99.020%,RSD 3.318%.The calibration of isoimperatorin was linear in range of 0.244～2.44 μg.The average recovery was 97.528%,RSD 2.904%.The average contents of imperatorin and isoimperatorin were 1.465 mg·g-1 and 0.575 mg·g-1 respectively.Conclusion The method is appropriate for the detemination of Radix Angelicae Dahuricae.

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Available abstract

Objective To develop a method for the determination of imperatorin and isoimperatorin in Radix Angelicae Dahuricae.Methods A HPLC method was established.Lichrospher-C18 column(250 mm×4.6 mm,5 μm)was used,with mixture of methanol and water in a gradient mode.The flow rate was 1.0 mL·min-1.Results The calibration of imperatorin was linear in range of 0.54～5.40 μg.The average recovery was 99.020%,RSD 3.318%.The calibration of isoimperatorin was linear in range of 0.244～2.44 μg.The average recovery was 97.528%,RSD 2.904%.The average contents of imperatorin and isoimperatorin were 1.465 mg·g-1 and 0.575 mg·g-1 respectively.Conclusion The method is appropriate for the detemination of Radix Angelicae Dahuricae.

Key concepts: Imperatorin, Radix (gastropod), Chemistry, Chromatography, High-performance liquid chromatography, Biology, Botany

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Determination of contents of imperatorin and isoimperatorin in Radix Angelicae Dahuricae from different areas by HPLC. — Research Paper | ScholarLens