Structure and Expression of GhCCR1 from Gossypium hirsutum L.

Primers were designed according to the cDNA sequence of cotton GhCCR1.PCR method was used to clone GhCCR1 from cotton and the expression of GhCCR1 in fibers at different developmental stages was analyzed by semi-quantitative RT-PCR.A length of 1 161 bp from genomic DNA of GhCCR1 was cloned by PCR.The genomic DNA of GhCCR1 contains four exons and three introns.Analysis of these introns indicated that they were rich in AT.All exon/intron junctions of the gene contain the gt/ag consensus splicing site.Semi-quantitative RT-PCR analysis revealed that GhCCR1 was expressed during different fiber developmental stages,and reached to the peak of expression in 20 days post anthers(DPA) fiber cells,suggesting that GhCCR1 may be involved in regulating fiber elongation and secondary wall thickening.