2011•Acta Agriculturae Boreali-SinicaRequires access
Structural and Expression Analysis of GhCAD1 Gene from Gossypium hirsuturm L.
Fan Ling
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Abstract
According to the cDNA sequence from cotton GhCAD1 gene,primers were designed.PCR method was used to clone GhCAD1 gene and the expression of GhCAD1 gene in different tissues of cotton was analyzed by semi-quantitative RT-PCR.A length of 2 898 bp sequence from genomic DNA of GhCAD1 was cloned by PCR.The genomic DNA of GhCAD1 contains five exons and four introns.Analysis of these introns indicated that they are rich in AT.Furthermore,a SBF-1 factor binding site was identified in the first intron of GhCAD1.Semi-quantitative RT-PCR analysis revealed that GhCAD1 can be expressed in different kinds of cotton tissues,and its mRNA accumulated most abundantly in leaf.GhCAD1 expression in cotton tissues was leafboll shellbractpetalrootcotyledon.