Study of Regulatory Effects of Jidesheng Sheyao on Cellular Immune Function of Mice

Objective:To study the regulatory effects of Jidesheng Sheyao tablets(JDS),a Chinese tradilional medicine,on cellular immune function of mice.Methods: Forty-eight BALB-c mice were randomly divided into four groups(12 mice in each group): the normal control group,the model group,treatment group 1 and treatment group 2.All experimental mice except those of the normal control group were administered with the Cyclophosphmide(CTX)(30 mg/kg,iH)for seven days.From the eighth day,JDS were given by intragastric gavage in every group twice every day,for lasting fourteen days.The normal control group and the model group were given the same volume of normal saline,the mice of the other experimental groups were respectively treated with corresponding drug.All animals were all picked eyeball to obtain blood,separation of serum,sterile separation of mouse spleen T lymphocytes;MTT assay was used for determination of NK cell activity;serum interferon-γ(IFN-γ),interleukin-2(IL-2) interleukin-4(IL-4) concentration were detected by enzyme-linked immunosorbent assay(ELISA).Results: The treatment group for NK cell killing activity was higher than the model group;treatment group in secretion of cytokines IL-2,IFN-γ levels in serum were significantly higher than those in model group.Conclusions: JDS sheyao tablet can increase the cell immune functions in immune-suppressed mice.