MOBILIZATION OF AUTOLOGOUS PERIPHERAL BLOOD STEM CELLS BY MITOXANTRONE-BASED BOMBINATIVE CHEMOTHERAPY AND HEMATOPOIETIC GROWTH FACTOR MOBILIZATION REGIMENS

Objective:To observe the efficacy of mitoxantrone based combinative chemotherapy and hematopoietic growth factor on autologous peripheral blood stem cells(APBSC) mobilization.Methods:MA mobilization regimen MOED mobilization regimen granulocyte colony-stimulating factor (G CSF) or G CSF and granulocyte macrophage colony stimulating factor (GM CSF) each,subcutaneously injected from the day of WBC recovery from nadir to the day of APBSC harvesting. APBSC harvesting started when WBC recovery to 2.5×10 9/L and CD + 34 cells percentage 1 % and finished when accumulated mononuclear cells(MNC)4×10 8/kg.CFU GM assay and CD + 34 cells detection of the APBSC were performed.Results:Twenty cases underwent the APBSC mobilization with mitoxantrone based chemotherapy combined with hematopoietic growth factor. Accumulated MNC for two successive days was(4.35±2.08)×10 8/kg,CD + 34 cells(9.87±4.30) ×10 6/kg and CFU GM(2.86±2.10)×10 4/kg. No severe toxicity was observed.Hematopoietic reconstitution was very well in 18 patients received the APBSC transplantation.Conclusion:Mitoxantrone based chemotherapy combined with hematopoietic growth factor was a safe and highly effective method for APBSC mobilization.