2007•Journal of Yangtze University Agricultural Science VolumeRequires access

Study on H_9N_2 Subtype Avian Influenza Virus by Monoclonal-antibody-Haemoglutination Inhibition Test

Zelin Wang

Abstract

25 strains H9N2 AIV were assayed through haemoglutination inhibition test(HI)by making use of 4 McAb(monoclonal antibody,McAb)to HA protein of H9N2-AIV and positive standard serum(anti-H9N2-AIV)respectively.The results showed that 23 of 25 strains H9N2 AIV reacted to the 4 McAb.The highest HI titer were 1∶212 for A McAb,1∶212 for B McAb,1∶212 for C McAb and 1∶212 for D McAb,respectively.The lowest titer were 1∶23 for A McAb,1∶29 for B McAb,1∶24 for C McAb and 1∶26 for D McAb respectively.However,2 of 25 strains H9N2 AIV had no HI reaction to all the 4 McAb,and their HI titers were 0.At the same time,the standard serum to H9N2 AIV could react to all the 25 strains H9N2 AIV.This indicated that 2 strains 17 and 18 H9N2 AIV strains had antigen variation in their HA.

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Key concepts: Titer, Monoclonal antibody, Avian influenza virus, Virology, Chemistry, Influenza A virus subtype H5N1, Molecular biology, Antibody