2007•Journal of Xinjiang Agricultural UniversityRequires access

Preparation and Identification of Monoclonal Antibody Against H5-AIV

Chen Hualan

Abstract

In this experiment,hybridoma cell strains were developed by fusion of SP2/0 mouse myeloma cells with spleen cells isolated from a BALB/C mouse which was immunized with the purified AIV DZJ/1100.The McAb cell strains were determined by indirect ELISA coated with recombinant NP protein of AIV and IFA.The specific results of H5 McAb,which react to H5-AIV by IFA method,suggest the McAb had a good specificity to H5 AIV and could be used abtain to the H5 subtype AIV.The subtypes of McAb belong to IgG1 and k strains.

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Key concepts: Monoclonal antibody, Spleen, Molecular biology, Recombinant DNA, Antibody, Cell fusion, Biology, Chemistry