The Expressions of Survivin and Smac in Epithelial Ovarian Tumor and Its Correlation

Objective To detect the expressions of Survivin and Smac mRNA and protein in epithelial ovarian tumor tissues,to investigate the clinical significance and analyze their correlation.Methods The Survivin and Smac mRNA expression levels from 20 cases of epithelial ovarian cancer and 20 cases of benign epithelial ovarian tumors were quantified by semi-quantitiative RT-PCR,and immunohistochemical staining(S-P method) was used to detect Survivin and Smac protein expressions in 60 cases with epithelial ovarian cancer and 60 cases with benign epithelial ovarian tumor.The relationship between Survivin and Smac protein expression was explored,and their relationship with relevant clinical pathologic characteristics were studied.Results The up-regulation of Survivin gene was observed in 70.0%(14/20) epithelial ovarian cancer tissues in comparison with the paired benign epithelial ovarian tumor tissues.Positive staining rate for Survivin was 61.67% in epithelial ovarian cancer tissues,while 6.7% positive staining in benign epithelial ovarian tumor tissues was(P0.05),and the expression of Survivin in epithelial ovarian cancer tissues was positively correlated with invasion depth,lymph node metastasis and differentiation(P0.05).Down-regulation of Smac gene was quantified in epithelial ovarian cancer tissues(40%) in comparison with the paired benign epithelial ovarian tumor tissues(90%).Positive staining rate for Smac was only 41.7% in epithelial ovarian cancer tissues,while 93.3% in benign epithelial ovarian tumor tissues(P0.05).The expression level of Smac was related to invasion depth,lymph node metastasis and differentiation(P0.05).Negative relationship was found between Survivin and Smac in epithelial ovarian cancer tissues(P0.05).Conclusion The expression of anti-apoptosis protein Survivin was significantly correlated with pro-apoptosis protein Smac,and both of them might play an important role in the occurrence and development of epithelial ovarian tumor.