2012•Zhongguo bingli shengli zazhiRequires access
Tetrandrine enhances radiosensitivity of human nasophayrngeal carcino- ma cell lines
Wu Xifu, Gehua Zhang, Jingjia Li, Huang Jiancong, Wang Kai, Yan Ruicheng, Zheng Jian, Xie Fengan, Jin Ye, Xian Liu
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Abstract
AIM:To study whether tetrandrine(Tet) enhances the radiosensitivity of human nasopharyngeal carcinoma cell lines in vitro and its mechanism.METHODS: The inhibitory effect on proliferation was evaluated by MTT assay.The radiosensitivity of the cells was compared by colony formation assay.The cell cycle was analyzed by flow cytometry.RESULTS: The maximum non-cytotoxic doses of Tet for CNE1 and CNE2 cells were 1.5 and 1.8 μmol/L,respectively.Compared with radiation group,the cell proliferation in Tet plus radiation group was significantly inhibited on the 4th to 6th days(P0.01).The mean lethal doses for CNE1 and CNE2 cells in radiation group were(1.26±0.02) Gy and(2.27±0.04) Gy,respectively,and the values changed to(0.73±0.05) Gy and(1.61±0.08) Gy in Tet plus radiation group,respectively,resulting in the sensitivity enhancement ratio of 1.73 and 1.40,respectively(P0.05).The CNE1 and CNE2 cells in G2 phase of the cell cycle in radiation group were(42.62±2.07)% and(34.82±2.74)%,respectively,while those in Tet plus radiation group were(17.02±1.87)% and(19.64±4.82)%,respectively.CONCLUSION: Tetrandrine enhances the radiosensitivity of human nasopharyngeal carcinoma cell lines and the mechanism may be related to the abrogation of radiation-induced G2 phase arrest.