2011•Zhonghua zhongyiyao xuekanRequires access

Effect of PolyphyllinI on Radiosensitivity in Nasopharyngeal Carcinoma Cell Line CNE-2 in Vitro

Hao Jiang

Abstract

Objective: To explore the radiosensitization of polyphyllin(PPⅠ) on nasopharyngeal carcinoma cell line CNE-2 and its possible mechanism.Methods: MTT assaywas used to measure the effect of PPⅠ on CNE-2 cell proliferation.Clonogenic assay was performed to determine the radiosensitizing effect of PPⅠ on CNE-2 cell line.Single-hitmultitargetmode was used to plot survival curve.The cell cycle and apoptosis were measured by flow cytometry.Results: PPⅠin a time-dose-dependent manner inhibited CNE-2 cell line proliferation,the IC50 of 24 and 48 hours were 6.68 and 3.84ug/mL respectively.The radiosensitization enhancement ratios(SERD0) of 1.5 and 3ug/mL PP Ⅰwere 1.10 and 1.44 respectively,and that was1.22 of DDP.The radiosensitization group had a higher apoptosis rate and G2 /M phase cells(P0.05).Conclusion: PPⅠ could enhance the radiosentivity of nasopharyngeal carcinoma cell line CNE-2,which possibly associated with inducing apoptosis and G2 /M phase arrest.

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Key concepts: Nasopharyngeal carcinoma, Radiosensitivity, Clonogenic assay, Apoptosis, Cell culture, Cell cycle, Flow cytometry, MTT assay