Research on radiosensitivity of nasopharyngeal carcinoma cell CNE1 in vitro to inhibitor of PI3K/Akt injection

Objective To evaluate whether Ly294002,suppressing phosphatidylinositol 3 kinase(PI3 K)/AKT survival signaling pathway,can change the sensitivity of nasopharyngeal carcinoma cell line CNE1 to radiotherapy.Methods The experiment was divided into control(A)group,simpledrug(B)goup,simple radiation(C)group and radiation plus drug(D)group.The cell survival curves were determined by cloning assay and cell clone technique for evaluating the cell lethal effects and by multitarget one-lit model and L-Q model.Results ①Experiment result of radiosensitvity indicated that the abmty of cell line CNE1 forming colony in the testting group degraded contrasting with A group.With the increasing ofradiation dose,forming colony ability decreased obviously.With the same radiation dose,colony rate in the D group was much less than that in the B group.The Do and Dq values were 0.783 Gy and 2.54 Gy in the B group,and in the D group,the Do and Dq were 0.522 Gy and 2.16 Gy.The SERDo was l.36.a/β value were 1.78 Gy and 9.65 Gy respectively,LY294002 can increase the radiosensitization of cell line CNE1.Conclusion LY294002 can increase the radiosensitization of nasopharyngeal carcinoma cell line CNE1.