2012Northern HorticultureRequires access

Study on Rapid Propagation by Leaf Tissue Culture of the Lycium barbarum L.

Liu Jin-feng

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Abstract

The leaf of Lycium barbarum L.was used as explants,induction of the callus and adventitious buds,differentiation,taking roots and transplant were studied.The results showed that the best media for inducing callus and adventitious buds was MS+NAA 0.2 mg/L+6-BA 1.0 mg/L;the best media for differentiation was MS+6-BA 0.3 mg/L+IAA 0.8 mg/L;the best media for inducing roots was 1/2MS+IBA 0.6 mg/L+NAA 0.2 mg/L.

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What this paper is about

The leaf of Lycium barbarum L.was used as explants,induction of the callus and adventitious buds,differentiation,taking roots and transplant were studied.The results showed that the best media for inducing callus and adventitious buds was MS+NAA 0.2 mg/L+6-BA 1.0 mg/L;the best media for differentiation was MS+6-BA 0.3 mg/L+IAA 0.8 mg/L;the best media for inducing roots was 1/2MS+IBA 0.6 mg/L+NAA 0.2 mg/L.

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Available abstract

The leaf of Lycium barbarum L.was used as explants,induction of the callus and adventitious buds,differentiation,taking roots and transplant were studied.The results showed that the best media for inducing callus and adventitious buds was MS+NAA 0.2 mg/L+6-BA 1.0 mg/L;the best media for differentiation was MS+6-BA 0.3 mg/L+IAA 0.8 mg/L;the best media for inducing roots was 1/2MS+IBA 0.6 mg/L+NAA 0.2 mg/L.

Key concepts: Lycium, Callus, Explant culture, Botany, Tissue culture, Biology, Murashige and Skoog medium, Chemistry

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