2007•Anhui nongye kexueRequires access
Tissue Culture and Plant Regeneration of Blackberry
LI Cong-yu
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Abstract
The leaves of the blackberry as the explant,and MS contained different concentration of 6-BA,NAA or IAA as the media,the callus induction and the plant regeneration of the blackberry were studied.The results showed that the calluses were induced from the leaf explants on the media of MS+6-BA 2.0-4.0 mg/L+NAA 0.01-0.5 mg/L and MS+6-BA 2.0-4.0 mg/L+IAA 0.01-0.5 mg/L.The induction rate was up to 100% on the media of MS+6-BA 4.0 mg/L+NAA 0.5 mg/L and MS+6-BA 4.0 mg+IAA 0.5 mg/L.Adventitious buds were induced from the calluses at the frequency of 49.7% on MS medium supplemented with 6-BA 2.0 mg/L and NAA 0.01mg/L.89.3% adventitious buds were rooted and induced regenerated plants on 1/2 MS medium supplemented with NAA 0.1 mg/L.