Research on the Plantlet Regeneration from Faba Bean Tissue Culture

[Objective] The effect of the different explants,media,genotypes and placement methods on the induction of adventitious bud and the plantlet regeneration of faba bean was studied.[Method] The immature embryo,immature cotyledon,mature cotyledon,mature cotyledon node as the experimental material,the effect of the different explants,media,genotypes and placement methods on the induction of adventitious bud and the plantlet regeneration of broad bean was studied in the regeneration system of the introduction of adventitious buds,the formation of individual small plant and its rooting.[Results] The results show that in the trial of four kinds of explants,the induction frequency of mature cotyledon culture was the highest,up to 95.0%.The medium suitable for the different explants culture was different.There was a big difference in the induction frequency from cotyledon culture to various genotypes of faba bean.The efficiency of the cotyledon vertically half-buried in the medium in its culture was better than that of it placed on the medium,which could produce higher induction rate of adventitious bud,and the adventitious buds could grow into a complete small plantlet with rooting system in the MS medium containing IBA with the concentration of 120 mg/L.[Conclusion] The process of organogenesis regeneration of faba bean was established through the experiment,which could provide a experimental basis for its further study.